Dissertation Defense: Carly Elizabeth Starke
Name: Carly Elizabeth Starke
Major: Microbiology & Immunology
Advisors: Jason Brenchley, Ph.D. and Marta Catalfamo, Ph.D.
Title: SIV-Specific CD8 + T Cells are Phenotypically, Functionally, and Clonotypically Distinct across Lymphoid and Mucosal Tissues
CD8 + T cell responses are necessary for immune control of simian immunodeficiency virus (SIV). However, the key parameters that confer antiviral potency remain elusive, feasibly because most studies to date have been restricted to analyses of circulating CD8 + T cells. We conducted a detailed clonotypic, functional, and phenotypic survey of SIV-specific CD8 + T cells across multiple anatomical sites in chronically infected rhesus macaques with high (> 10,000 copies/mL plasma) or low burdens of viral RNA (< 10,000 copies/mL plasma). No differences in response magnitude were identified across anatomical compartments. Rhesus macaques with low viral loads (VLs) harbored higher frequencies of polyfunctional CXCR5 + SIV-specific CD8 + T cells in various lymphoid tissues. Additionally, public Gag-specific CD8 + T cell clonotypes were also more commonly shared across distinct anatomical sites than the corresponding private clonotypes, which tended to form tissue-specific repertoires, especially in the peripheral blood and the gastrointestinal tract. Collectively, these data suggest that functionality and tissue localization are important determinants of CD8 + T cell-mediated efficacy against SIV.
Friday, April 5, 2019 at 10:00am to 12:00pm
Medical and Dental Building, NE301
3900 Reservoir Road, N.W., Washington